DNA

Part:BBa_K2440017

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:05, 31 October 2017 by MINXIAO (Talk | contribs) (→‎Usage and Biology)


miR-875 target sequence

It is the target sequence of miR-875, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.

Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1

In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. The expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

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Categories
Parameters
None