Coding
TSHHantag

Part:BBa_K2404001:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)
Revision as of 20:49, 30 October 2017 by Gparry75 (Talk | contribs) (→‎Design Notes)


A Thyroid Stimulating Hormone antagonist with His-Tags


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 795
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 827


Design Notes

We generated this part through the following steps:


- We ordered the following TSH gBlock from IDT
T--Cardiff_Wales--TSH_gblock.png

- This was introduced into pSB1C3 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning

Source

This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags. It was ordered as a g-block.

References