Coding
LIMS1

Part:BBa_I742110

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-19)
Revision as of 17:33, 25 October 2007 by Cfrench (Talk | contribs)

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LIMS1

Limonene synthase 1 from Citrus limon (accession AF514287). Synthesised by Geneart. Limonene synthase converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. Note: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG.

For a diagram of limonene synthesis, and the synthesis of other lemon flavour compounds see [http://2007.igem.org/Edinburgh/Yoghurt/Design#Lemon_Flavour_Production Edinburgh Yoghurt Wiki]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 100


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