Composite

Part:BBa_K2323004

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-26)
Revision as of 20:23, 30 October 2017 by Jorge Quintero (Talk | contribs)


Lwa Cas13a under T7 promoter with solubility tag

Uses a T7 promoter and RBS B0034 (BBa_K525998) to express Lwa Cas13a (BBa_K2323000) flanked by a 6x His-Tag to allow purification and a SUMO-tag to improve solubility. This is terminated by a Tphi terminator. The whole coding sequence is also available as BBa_K2323002.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1768
    Illegal BglII site found at 3448
    Illegal BamHI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2847


Usage and Biology

Cas13a from Leptotrichia wadei (Lwa)


[edit]
Categories
//awards/composite_part/nominee
Parameters
None