Part:BBa_I20260

Measurement Kit Test of J23101

Measkit test of J23101

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 706
  

Characterization IONIS_PARIS 2017

Group & Author: IONIS_PARIS /La Paillasse/2017

Summary: We sequence the sequence BBa_I20260, and we transformed it into pSB1C3. Method: See the bacterial transformation protocol in our wiki IONIS_PARIS 2017_Lab work_protocols.

Results:

The bacteria transformation worked, we had colonies and we then did a PCR colony (Figure 1) to verify if the GFP was transformed into pSBIC3. We picked different colonies and the positive result was observed in the well nº3 (GFP 1). In conclusion the GFP was successfully transformed into pSB1C3.

Figure 1: PCR colony. A) Ladder, B) mRFP protein generator, C) GFP 1, D) GFP 2, E)GFP 3.