Regulatory
Pu

Part:BBa_I723020:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)
Revision as of 17:17, 25 October 2007 by Scott.w.ramsay (Talk | contribs) (→‎Design Notes)

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Pu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter can go in front of any gene you want expressed in response to the presence of BTEX compounds, provided you also have the XylR+Pr component

Source

Cloned from the TOL plasmid from Pseudomonas putida MT2. This is the promoter that regulates the TOL catabolic plasmid upper pathway operon.

References

Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.

Harayama, S., Leppik, R.A., Rekik, M., Mermod, N., Lehrbach, P.R., Reineke, W., and Timmis, K.N. 1986. Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product. Journal of Bacteriology 167, 455-461.