Coding

Part:BBa_K2271124

Designed by: Fiona Edenhofer   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 15:34, 30 October 2017 by PhilippMueller (Talk | contribs)


ValS PTS1

Valencene synthase converts FPP into Valencene. We chose the ValS from Callitropis nootkatensis, because other valencene synthases perform relatively inefficient in microorganisms. For example, they show a relatively poor product specificity, are less efficient in producing sesquiterpene than other synthases and have a significant amount of side products like germacrene A. In contrast to this, ValS from C. nootkatensis has a good product specificity and is more robust in terms of pH and temperature changes. It has also a better yield than citrus valencene synthases in yeast.

This part additionally holds a PTS1 sequence for peroxisomal PEX5 import. For cytosolic expression please choose part: Bba_K2271118

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
    Illegal BamHI site found at 2732
    Illegal XhoI site found at 2768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None