Composite

Part:BBa_K2336036:Design

Designed by: Ziyang Xiao   Group: iGEM17_HUST-China   (2017-10-14)
Revision as of 15:16, 30 October 2017 by Xzy1012 (Talk | contribs) (→‎Design Notes)

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PmrC-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 397
    Illegal XhoI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1085


Design Notes

In order to replace GFP more conveniently, we respectivey added HindIII and SalI at both ends. Also, to avoid cutting pET28a, which may influence our construct. we changed SalI into XhoI.

Source

Synthesized by IDT.

References