Composite

Part:BBa_K2201273

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 13:28, 30 October 2017 by Svinke (Talk | contribs)

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Reversed mRFP under T3-polymerase control for signal strengthening

Reversed mRFP under T3-promoter control. This part could be used as a highly specific reporter in complex genetic cirsuits. The opposite direction of the CDS prevents basal transcription. Furthermore the T3 and T7 polymerase are orthognal, thus this reporter could be used orthgonal to the expression with the T7-polyerase in E. coli strains like BL21. The characterisation of this part could be found on the page of the composite part BBa_K2201373.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
    Illegal AgeI site found at 147
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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