Coding

Part:BBa_K2333011:Design

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-10-27)
Revision as of 13:28, 30 October 2017 by Cemonette (Talk | contribs) (→‎Design Notes)


mf-Lon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1834
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 2124
    Illegal AgeI site found at 2149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this part was adapted from Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease". It was codon-optimized for E. coli.

Source

meso

References