Part:BBa_K2336036

PmrC-GFP

This part is used to detect whether the sequence is expressed successfully.

Usage and Biology

PmrC is a promoter used to start the composite. GFP is green fluoresent protein gene.

The aim that we design this part is to get prepared for the material, then PmrA-PmrB(LBT) can be linked to it. Because we need a large amount of this part, we standardize this part independently. Actually, this part is not a functional part. It a basic to get more parts and construct the whole circuit. Figure 1-1 shows the circuit of PmrC-GFP.

Figure 1-1: The circuit of PmrC-GFP.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 397
  Illegal XhoI site found at 436
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1162
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1085