Composite

Part:BBa_K2323000:Design

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)
Revision as of 12:50, 30 October 2017 by AuroreD (Talk | contribs) (→‎References)

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Lwa Cas13a + Tphi terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 2966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2365


Design Notes

The protein is quite large, and therefore the sequence should be codon-optimized for the organism and strain it will be expressed in. We checked that there was no excessive use of rare codons for expression in E.coli, and used the strain Rosetta2 which contains an additional plasmid for rare codons.


Source

Leptotrichia wadei

References

J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)