Part:BBa_K2440006

Synthetic PA

The Poly A, also called Polyadenylation, is the addition of a poly adenine bases tail to a messenger RNA. It is important for the nuclear export, translation, and stability of mRNA. ¹

Usage and Biology

The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. ^2-3

At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] Proudfoot NJ, Furger A, Dye MJ. (Feb 2002). "Integrating mRNA Processing with Transcription". Cell. 108(4):501-12. PMID: 11909521

[2] Guhaniyogi J, Brewer G. (Mar 2001). "Regulation of mRNA stability in mammalian cells". Gene.265(1-2):11-23. PMID: 11255003

[3] Richter JD. (Jun 1999). "Cytoplasmic Polyadenylation in Development and Beyond". Microbiol Mol Biol Rev. 63(2):446-56. PMID: 10357857 PMCID: PMC98972