Coding

Part:BBa_K2336026

Designed by: Ziyang Xiao   Group: iGEM17_HUST-China   (2017-10-14)
Revision as of 12:35, 30 October 2017 by Liziyi (Talk | contribs)


oprF-GS-FLAG-LBT1-GS-LBT1-GS-LBT1

The report part of our circuit. Oprf can be expressed as an anchor on the cell membrane to make sure the LBT can combine with terbium (Tb3 +) on the surface of the bacteria.

Characterization

This is a part for recycling.LBT1（lanthanide binding tag）can bind the lanthanide ions and it can be expressed at the cell membrane of E.coli with the help of oprF.We set the 'FLAG' between oprF and LBT for fluorescence detection.In our experiment,we let the LBT1 be expressed successfully which means that the lanthanide ions in the water would be bound and recycled effectively after we put the in the water.

Figure1-1:oprF-LBT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
    Illegal NheI site found at 749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 933
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None