Tag

Part:BBa_K2323011:Design

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-27)
Revision as of 11:52, 30 October 2017 by AuroreD (Talk | contribs) (→‎Source)

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6xHis/Twin strep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is convenient for His purification. However, keep in mind that tags affect protein functionality. It is better to have a cleavable protein tag between this tag and the protein coding sequence. See for example BBa_K1319001

Source

Sequence was taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)

References