Composite

Part:BBa_I723021

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)
Revision as of 16:41, 25 October 2007 by Scott.w.ramsay (Talk | contribs)

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XylR transcriptional regulator

Contains the XylR gene (encodes a transcriptional regulator activated by xylene) under the control of its native promoter. To complete the sensor, clone the gene you want to induce under the control of promoter Pr.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 530
    Illegal BglII site found at 1992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1183


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