Deleted
Sample Discontinued
Experience: Works
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Part:BBa_K2371016

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-24)
Revision as of 11:23, 30 October 2017 by Joyce324 (Talk | contribs)

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C-Fluc-dCas9

dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. BGIC-Union split the luciferase into two separate parts(NFluc:BBa_K1689010 and CFluc:BBa_K1689009) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can emit luminescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1689
    Illegal NheI site found at 4876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 30
    Illegal BamHI site found at 3968
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 353
    Illegal AgeI site found at 492
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None