Coding

Part:BBa_K2361000

Designed by: Mart Bartelds   Group: iGEM17_Groningen   (2017-10-09)
Revision as of 08:54, 30 October 2017 by Mart (Talk | contribs)


spdCas9

This part contains the protein coding sequence for the dCas9 originating from Streptococcus pyogenes. This protein is a catalytically-dead Cas9 variant, which lacks endonuclease activity. It is used for gene repression using CRISPR-interference.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3379
    Illegal XhoI site found at 4115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/crispr/cas9
Parameters
biology
protein