Part:BBa_K2276007:Experience

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Applications of BBa_K2276007

We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it.Due to the limit of the expriment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately.Here is the result.

File:SCU-China 2017 RBS fluorescence intensity over time .png

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Figure 1 The fluorescence intensity changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

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Figure 2 The OD600 changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

   We can see that Part:BBa K2276007 has a much strong expression than Part:BBa_I13521.

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