Part:BBa_K2440007

RNF8 3-UTR

This is the 3’prime untranslated region of RNF8 gene. It is the section of mRNA that immediately follows the translation termination codon and contains regulatory regions that post-transcriptionally influence gene expression. ¹.

Usage and Biology

RNF8 gene is a human gene which encodes an enzyme called E3 ubiquitin-protein ligase RNF8. ²

RNF8 plays an important role in the immune system and in the DNA repair process. ³

The RNF8 protein contains a RING finger motif and an FHA domain. It could interact with several class II ubiquitin-conjugating enzymes and participate in the ubiquitination reaction of certain nuclear proteins. ⁴

There are 3 DNA repair pathways by which RNF8 promotes the repairing of DNA damage: homologous recombinational repair (HRR), non-homologous end joining (NHEJ), and nucleotide excision repair (NER). ⁴⁵⁶

The double-strand break in DNA could be repaired by either HRR and NHEJ. While the pyrimidine dimers, which are induced by UV, could be repaired by NER. ⁵⁶⁷

The depletion of RNF8 protein causes cell growth inhibition and cell cycle arrest. ⁸

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1435
Illegal XbaI site found at 3
Illegal PstI site found at 637
Illegal PstI site found at 2007
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1435
Illegal PstI site found at 637
Illegal PstI site found at 2007
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1435
Illegal BglII site found at 927
Illegal BglII site found at 1755
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1435
Illegal XbaI site found at 3
Illegal PstI site found at 637
Illegal PstI site found at 2007
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1435
Illegal XbaI site found at 3
Illegal PstI site found at 637
Illegal PstI site found at 2007
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1116