Part:BBa_K2440004:Design

hAgo2

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 24
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1170
  Illegal XhoI site found at 2080
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 33
  Illegal AgeI site found at 1868
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 730
  

Design Notes

None.

Source

We obtain it from PCR.

References

[1] Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T.(2004 Jul). "Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs". Mol Cell. 23;15(2):185-97. PMID: 15260970 DOI: 10.1016/j.molcel.2004.07.007

[2] Hutvagner G, Simard MJ. (2008). "Argonaute proteins: key players in RNA silencing". Nature Reviews Molecular Cell Biology. 9 (1): 22–32. DOI: 10.1038/nrm2321

[3] Tang G. (Feb 2005). "siRNA and miRNA: an insight into RISCs.". Trends in Biochemical Sciences. 30 (2): 106–14. PMID 15691656. DOI: 10.1016/j.tibs.2004.12.007.

[4] Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. (Jul 2004). "Human Argonaute2 Mediates RNA Cleavage Targeted by miRNAs and siRNAs". Molecular Cell. 15 (2): 185–197. PMID 15260970. DOI: 10.1016/j.molcel.2004.07.007.

[5] Ronemus M, Vaughn MW, Martienssen RA. (2006 Jul). "MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis". Plant Cell. 18(7):1559-74. Epub 2006 Jun 23. PMID: 16798886 PMCID: PMC1488920 DOI: 10.1105/tpc.106.042127