Composite

Part:BBa_K2382013

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 03:57, 30 October 2017 by Herlohuang (Talk | contribs) (→‎Usage and Biology)

EAAAK+ RFP+Histag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.

This is also an improvement of previous BioBrick Part (BBa_E1010). It encodes an RFP that have the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein with this part may have red color and the ability to be purified easily.

Characterization of theEAAAK+ RFP+Histag

References

[edit]
Categories
//cds/reporter/rfp
Parameters
None