Composite

Part:BBa_K2382005

Designed by: TING-YU LIN   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 02:05, 30 October 2017 by Herlohuang (Talk | contribs) (→‎Characterization of the Thioredoxin with polylinker)

Thioredoxin-FGD fusion protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1117
    Illegal BamHI site found at 405
    Illegal XhoI site found at 411
    Illegal XhoI site found at 1383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal NgoMIV site found at 975
    Illegal AgeI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

By ligating these two different parts as a fusion protein, it is supposed to raise the solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).


Characterization of the Thioredoxin-FGD fusion protein

References

Carsten Berndt, Christopher Horst Lillig, Arne Holmgren, Thioredoxins and glutaredoxins as facilitators of protein folding, In Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1783, Issue 4, 2008, Pages 641-650, ISSN 0167-4889, https://doi.org/10.1016/j.bbamcr.2008.02.003. (http://www.sciencedirect.com/science/article/pii/S0167488908000700)

[edit]
Categories
//cds/biosynthesis
Parameters
None