Reporter

Part:BBa_K2333437

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-10-27)
Revision as of 22:54, 29 October 2017 by Chli (Talk | contribs)


Copper sensor with Protein Degradation Tag A

This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of protein degradation tagged RFP from the downstream No part name specified with partinfo tag. promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1229
    Illegal AgeI site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None