Coding
Sialidase

Part:BBa_K2235005

Designed by: Shanlin Tong, Shivashree Dhanaraj, Sina Amoor Pour and Gilai Nachmann   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 21:08, 29 October 2017 by Shiva (Talk | contribs)


Sialidase enzyme

Usage and Biology

Sialidase hydrolyses glycosidic linkages of terminal sialic acid residues in glycoproteins. In other words, it removes the sialic acids from the ends of the mucin constructs. This will help our bacteria to interact with the mucins for further degradation of the saccharides. Additionally, the removal of the sialic acids results in an exposure of other sugar groups on the mucins to other enzymes. Thus, the removal of sialic acids may result in even further mucus degradation by bacteria using different enzymes.

Figure 1: Schematic representation of Sialidase enzyme reaction mechanism.



More details to the characterization of Sialidase can be found in the composite part BBa_K2235009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 577
    Illegal NgoMIV site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1047


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None