DNA
Part:BBa_K2323013:Design
Designed by: Aurore Dupin Group: iGEM17_Munich (2017-10-29)
Spacer between His tag and coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There's no special considerations here. It's a spacer sequence. It should be a multiple of 3 nucleotides, to not shift the reading frame.
Source
random DNA sequence