Coding

Part:BBa_K2271103

Designed by: Jan Maika and Philipp Rink   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 19:36, 29 October 2017 by Philipp R (Talk | contribs)


pex15

This part contains a truncated version of the peroxisomal membrane protein pex15 (315-383). The part can be used as a peroxisomal membrane anchor on either its C- or N-terminus.

P: This part is a truncated version of the S. cerevisiae Pex15 which is a membrane peroxisomal membrane Protein. This version could be used as a membrane anchor for applications at the peroxisomal membrane. Both C- and N-Terminus could be used for a fusion. The N-Terminus is located in the cytoplasm and the C-Terminus in the peroxisomal lumen.

The functionality of this part was validated by microscopy. For the microscopy mVenus was fused to the C-Terminus of Pex15. We could observe a peroxisomal typical localisation in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None