Coding

Part:BBa_K2271060:Experience

Designed by: Philipp Rink and Jan Maika   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 18:49, 29 October 2017 by Jan-maika (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2271060

For testing this part we used a fusion with the N-Terminus of a Peroxisomal Membrane anchor. We co-expressed this construct with Gus-PTS to perform a GUS Assay.(oder: We performed a GUS-Assay by targetting GUS(beta-Glucuronidase) to the Peroxisome using a GUS-PTS1.) The secreted GUS in the supernatant was measured with the turnover of 4-methylumbelliferyl-beta-D-glucuronide to 4-methyl umbelliferone (4-MUG). The fluorescent 4-MUG was measured with a plate reader (excitation: 365 nm, emission: 465 nm). Different peroxisomal membrane anchors were tested using the GUS-Assay. The highest activity of GUS could be measured in the supernatant of Pex15 (Link to part) as a Membrane Anchor.

User Reviews

UNIQce001aedb7a5cd1f-partinfo-00000000-QINU UNIQce001aedb7a5cd1f-partinfo-00000001-QINU