Measurement

Part:BBa_K2333407

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-06-29)
Revision as of 18:38, 29 October 2017 by Sdhawan (Talk | contribs)


UNS J23100 sfGFP pdt A

This part is designed to facilitate easy measurement of the strength of protein degradation tag (pdt) A by measuring time to steady-state fluorescence values of sfGFP under the control of the strong constitutive promoter J23100. William and Mary iGEM 2017 used pdts as a method to control gene expression speed.This part was utilized to characterize the degradation properties of pdt A and confirm the fact that different pdts have different degradation strengths. See [http://2017.igem.org/Team:William_and_Mary/Results William and Mary's 2017 project] for more details This part is one of a series of easy sfGFP reporter pdt parts. Series range is from BBa_K2333401 to BBa_K2333406


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 106


[edit]
Categories
Parameters
None