Composite

Part:BBa_K2232014

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-18)
Revision as of 18:19, 29 October 2017 by James zhang (Talk | contribs)


P43_TSLV1-CA-alpha_rrnBT1

For facilitating the interconversion of CO2 and HCO3− which is beneficial to the precipitation of CaCO3, a plasmid containing Carbonic anhydrase coding gene have built and transferred into B. subtilis strain WB800 and express successfully.The promoter P43 used in the plasmid is a strong promoter in Bacillus subtilis 168,and the Carbonic anhydrase is from The polyextremophilic bacterium Bacillus halodurans TSLV1 (MTCC 10961, 16S rDNA Acc. No. HQ235051) which belong to α-type in classes of CAs. The terminator we used is rrnBT1(BBa_B0010) from E. coli rrnB, which will stop the Transcription of gene and raise the efficiency of expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NotI site found at 1138
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 376
    Illegal SapI.rc site found at 534
    Illegal SapI.rc site found at 846


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Categories
Parameters
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