Measurement

Part:BBa_K2333413

Designed by: Sejal Dhawan   Group: iGEM17_William_and_Mary   (2017-10-27)
Revision as of 17:26, 29 October 2017 by Chli (Talk | contribs)


UNS J23100 mScarlet-I

This part is contained in a suite of protein degradation tagged mScarlet reporters under the control of the strong constitutive promoter J23100. These parts, in combination with inducible mf-Lon protease constructs, allowed us to characterize the degradation properties of each protein degradation tag (pdt) on a plasmid-based system. We successfully demonstrated distinct levels of protein degradation by each of the 6 pdt’s, and mScarlet reporters have been codon-optomized for E. coli and feature a double stop codon for enhanced efficiency. This specific part is a tagless control construct (J23100 mScarlet with no pdt) which can be used as a comparison against protein degradation for parts with pdt's.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
    Illegal NotI site found at 605
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None