Generator

Part:BBa_K2207011

Designed by: Yuxing Chen   Group: iGEM17_ZJU-China   (2017-10-29)
Revision as of 17:03, 29 October 2017 by Registry (Talk | contribs)

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phlO-mRFP

BBa_K2207011 generator(phlF regulated) This part is an inducible generator regulated by PhlF. It contains an inducible promoter K2207010 and a biobrick E1010.

In the absence of PhlF protein, this part can turn the yeast Saccharomyces cerevisiaea red. In the presence of PhlF protein, this part fails to turn the yeast Saccharomyces cerevisiaea red. PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG). PhlF can be coded by BBa_K2207009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1384
    Illegal AgeI site found at 1496
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None