Coding

Part:BBa_K2382002:Design

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 16:47, 29 October 2017 by Herlohuang (Talk | contribs)

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F420-Dependent Glucose-6-phosphate Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 695
    Illegal XhoI site found at 961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 553
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have the coden optimized for E. Coli BL21.

Source

Mycobacterium smegmatis str. MC2 155 https://www.ncbi.nlm.nih.gov/nuccore/118467340/