Coding

Part:BBa_K2382007

Designed by: HO-LO HUANG   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 16:20, 29 October 2017 by Herlohuang (Talk | contribs) (→‎References)

Anti-aflatoxin scFv (with start coden)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 342
    Illegal SapI.rc site found at 255


Usage and Biology

Since bacteria can’t produce the whole antibody, we decide to use scFv to replace it. It contains two polypeptide chains linked by a linker. And we add a start coden (ATG)

Characterization of the Anti-aflatoxin scFv (with start coden)

References

(1) Li, X., et al., Molecular characterization of monoclonal antibodies against aflatoxins: a possible explanation for the highest sensitivity. Anal Chem, 2012. 84(12): p. 5229-35.

(2) Chen, X., Zaro, J. L., & Shen, W. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357-1369. doi:10.1016/j.addr.2012.09.039

[edit]
Categories
//cds/receptor/antibody
//collections/immune_regulation/antibodies
Parameters
proteinAnti-aflatoxin scFv (with start coden)