Coding

Part:BBa_K2382004

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 12:12, 29 October 2017 by Herlohuang (Talk | contribs)

Thioredoxin with polylinker


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 367
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

This part previously functioned as a DNA recombination and repair protein in E. coli. It is also found that Thioredoxin is capable of increasing the solubility of our protein, MSMEG5998. Therefore, we create this part for future igem teams who want to make their protein more soluble when facing inclusion bodies in E. coli.

Characterization of the Thioredoxin with polylinker

References

[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//proteindomain/linker
Parameters
functionenzyme joint in reductionoxidation reaction