Composite

Part:BBa_K2203006

Designed by: Lisa Dratva   Group: iGEM17_EPFL   (2017-10-25)
Revision as of 11:24, 29 October 2017 by Melekcabani (Talk | contribs)


HepHCV_ToeholdB

This composite part consists of a T7 promoter (BBa_J64997) in front of the toehold, a lacZalpha coding sequence (BBa_I732006) downstream of it and a T7 terminator (BBa_K731721) at the end of it. An RNA toehold switch with lacZalpha as a reporter added after the switch. When the trigger sequence in form of RNA or ssDNA is present in the sample, the toehold will unfold and lacZalpha will be translated. For more information about toeholds, go to 2017.igem.org/Team:EPFL

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None