Coding

Part:BBa_K1033121

Designed by: Alexander Virtanen   Group: iGEM13_Uppsala   (2013-09-11)
Revision as of 09:51, 29 October 2017 by Yaakovbul (Talk | contribs)

Miraculin

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.


Part improvement: team Tel-Hai 2017

Group: Tel-Hai 2017

Author: Almog Angel


BBa_K2408023


Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features:
1. ADH1 - strong constitutive promoter for S. cerevisiae.
2. His tag for purification
3. ADH1 terminator for more effective expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None