Composite

Part:BBa_K2374007:Design

Designed by: Jianfeng Zhou   Group: iGEM17_Tongji_China   (2017-10-26)
Revision as of 08:09, 29 October 2017 by Zjeng (Talk | contribs) (→‎Design Notes)


UAS-TH -> (fruit fly)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1540
    Illegal XhoI site found at 672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 918
    Illegal BsaI site found at 1884
    Illegal BsaI.rc site found at 636
    Illegal BsaI.rc site found at 1402
    Illegal BsaI.rc site found at 1551


Design Notes

At the forward of the ple sequence, there is a ~150bp region which contains up to 5 copies of some specific sequences. This makes it difficult to design specific primers to clone this gene. We have tried many different sets of primers and different DNA Polymerases to clone ple from Drosophila's cDNA library. Unfortunately we all failed to do this. There had been appeared some negative fragments which even had the same size of the real one.

Source

DROSOPHILA

References