DNA

Part:BBa_K2492004

Designed by: ZHOU Xinyu   Group: iGEM17_SUSTech_Shenzhen   (2017-10-09)
Revision as of 06:25, 29 October 2017 by X Maze (Talk | contribs)

pODR-10: the promoter of coding odorant diacetyl receptor protein.

Part BBa_K2492004 contains odr-10. It is a section of genomic DNA acts as a promoter. In C.elegans, ODR-10 encodes a large family of seven transmembrane olfactory receptor genes[1]which is specifically expressed in neuron AWA and affect chemotaxis to the volatile diacety. POrd-10 is a section of genemoic DNA in front of Ord-10 genes promoting expression of Ord-10 genes[2]. AWA is a member of the olfactory neurons [2]. It responses to attractive volatile odorants. Since we expect to express the functional channelrhodopsins and indicator in specific neuron(AWA for instance), we use pODR-10 to construct plasmid. If CoCHR is expressed in AWA, we are capable of activating it to manipulate the state of AWA. In our project, we integrated ord-10::Chrimson::GEM-GECO::mCherry transgene to C.elegans by microinjection. Thus, CoCHR (Part BBa_K2492001:CoCHR) promoted by ord-10 is expressed in AWA. We can enhance the attraction of C.elegans through the control of AWA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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