Coding
Part:BBa_K2267011
Designed by: HE, BOTAO Group: iGEM17_TUST_China (2017-10-25)
Revision as of 05:19, 29 October 2017 by Carry zhang (Talk | contribs)
SpyTag
SpyTag is a protein, which can form irreversible bond with SpyCatcher. Two coding sequences, Escherichia coli RBS and 6*His tag, are added in this part; thus, provides specific purification. Originated from Streptococcus pyogenes fibronectin-binding protein FbaB, the SpyTag-SpyCatcher system is commonly used for binding, labeling, immobilization and creating new kinds of application. More application can be created by adding function protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |