Coding

Part:BBa_K2267011

Designed by: HE, BOTAO   Group: iGEM17_TUST_China   (2017-10-25)
Revision as of 05:19, 29 October 2017 by Carry zhang (Talk | contribs)

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SpyTag

SpyTag is a protein, which can form irreversible bond with SpyCatcher. Two coding sequences, Escherichia coli RBS and 6*His tag, are added in this part; thus, provides specific purification. Originated from Streptococcus pyogenes fibronectin-binding protein FbaB, the SpyTag-SpyCatcher system is commonly used for binding, labeling, immobilization and creating new kinds of application. More application can be created by adding function protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None