Generator

Part:BBa_K2486004

Designed by: Cauă Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-22)
Revision as of 23:35, 28 October 2017 by Gabriellovate (Talk | contribs)


dtxR generator

A part that constitutively generates the DtxR repressor.

Usage and Biology

BBa_J23108 BBa_B0034 BBa_K2486024 BBa_K2486005 BBa_K2486025 BBa_K2486006 The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

  1. A promoter (BBa_J23108) from Anderson promoter collection.
  2. A ribosome binding site (BBa_B0034).
  3. A short spacer of 6bp (BBa_K2486024).
  4. dtxR gene (BBa_K2486005).
  5. A short spacer of 16bp (BBa_K2486024).
  6. A synthetic terminator (BBa_K2486006).

We built this part in order to constitutively inhibit our synthetic promoter, the PdtxR wich is best described in parts (BBa_K2486023), (BBa_K2486014) and (BBa_K2486009).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None