Part:BBa_K2289000

STX Aptazyme J_1

This part is a noncoding single-stranded DNA Aptazyme, this molecule combines 3 modules, an HRP-mimicking DNAzyme, an aptamer for recognition of Saxitoxin (related to the red tide) and linkers.

Aptazyme: An aptazyme is a ssDNA molecule thay can recognize a ligand and catalyze a reaction. The funcionality of this molecule depends on three modules; an aptamer, for recognition of the ligand, a DNAzyme, for catalize the reaction and linkers, fundamental parts that can bind specific zones of the Aptazyme, and with that those can act as kidnappers of the catalytic zone of the molecule. The linkers are fundamental, because they are placed in such a way that when the aptamer is not interacting with the ligand, the linkers are kidnapping the DNAzyme, altering the conformation of the DNAzyme (see Figure 1a).

When the aptamer interacts with the ligand, a conformational change takes place in the molecule, this conformational change promote the movement of the linkers from the original place, and in consecuense the DNAzyme is released, with that the DNAzyme can take the catalytic conformation and can catalyse the reaction (see Figure 1b).

This part consists on an Aptazyme that can recognise Saxitoxin (STX) and that can catalyse a chromogenic reaction.

Modules:

STX M30f Aptamer (<Part:BBA_K2289004 href=”https://parts.igem.org/Part:BBa_K2289004”> </a>)

HRP-Mimicking DNAzyme (<Part:BBa_K1614007 href=”https://parts.igem.org/Part:BBa_K1614007”> </a>):

J_X linker:

All in one:

Primal fold:

In the presence of the toxin:

Dynamics:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]