Regulatory

Part:BBa_K2448011

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 21:41, 28 October 2017 by Brumake (Talk | contribs)

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pPsiR from Agrobacterium tumefaciens

This part is the promoter region (0.36 kb upstream) of the PsiR gene of Agrobacterium tumefaciens str. C58 (Atu4743)

pPsiR is predicted to be repressed by the PsiR transcription factor (BBa_K2448006) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiR promoter together with the Agrobacterium tumefaciens PsiR transcription factor (BBa_K2448006) to build a Psicose biosensor (BBa_K2448026). The results show that pPsiR is functional in E. coli and that it behaved as predicted under and without induction. 

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    299 LDF-  3.13
-10 box at pos.    284 tggtgcaat Score    40
-35 box at pos.    264 ttgcca    Score    61
Oligonucleotides from known TF binding sites:
    rpoD17:  TGCAATAA at position     287 Score -   5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None