Coding
Part:BBa_K2271105
Designed by: Manuel Lentzen Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
PEX5 variant R15
PEX5
Brief introduction
PEX5 is one of two proteins that mediate most of the protein import into the peroxisomal matrix. It recognizes the very c-terminal peroxisomal targeting signal 1 (PTS1), then folds itself to a more compact version and interacts with a variety of enzymes.
The whole process is divided into five steps: binding, transport, docking, translocation and receptor recycling.
Variant
We followed a targeted mutagenesis approach to achieve an orthogonal import mechanism − our PEX5 variant should recognize a non-native PTS1 variant which is not recognized with the wildtype PEX5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 409
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |