Composite

Part:BBa_K2455007:Design

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-27)
Revision as of 18:42, 28 October 2017 by Jon UCPH (Talk | contribs) (→‎References)


YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1876
    Illegal BglII site found at 1937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
    Illegal AgeI site found at 639
    Illegal AgeI site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

The YddG gene is a synthetic codon optimized version of the E. coli MG1655 YddG gene.

The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (Gu et al., 2012), in addition a hexa his-tag has been added.

References

Gu, P. et al. (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories, 11(1), p.30.

Wang, J. et al. (2013). Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Applied Microbiology and Biotechnology, 97(17), pp.7587-7596.