Coding

Part:BBa_K2448009

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 16:26, 28 October 2017 by Brumake (Talk | contribs)


FruR from Escherichia coli

This part is the FruR found in Escherichia coli K-12 (ECK0081). The sequence was modified

Usage and Biology

FruR is transcriptional repressor-activator for carbon metabolism. It activates transcription of genes involved in Krebs cycle, glyoxylate shunt and gluconeogenesis and represses genes involved in sugar catabolism, such as FruB.

Our team used this transcription factor together with the FruB promoter (pFruB, BBa_K2448017) to build a Fructose biosensor (BBa_K2448032) and the results shows that FruR behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None