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Part:BBa_K2448001
mEmerald
mEmerald is a derivative of the Green Fluorescent Protein from Aequorea victoria with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains six mutations compared to wild-type: F64L; S65T; S72A; N149K; M153T; I167T; A206K [2].
This protein has been specifically optimized for Escherichia coli K12 with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
Usage and Biology
Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.
Our team used this codon optimized mCherry to build the Universal Biosensing Chassis (BBa_K2448023).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
None |