Coding

Part:BBa_K2455004

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-27)
Revision as of 13:55, 28 October 2017 by Jon UCPH (Talk | contribs)


YddG E. coli Tyr, Trp, Phe exporter

YddG is an aromatic amino acid exporter that is responsible for the secretion of Tryptophan, Tyrosine and Phenylalanine.

Previous work by [https://link.springer.com/article/10.1007%2Fs00253-013-5026-3 Wang et al. have shown that over-expression of YddG in E.coli increases the accumulation of Tryptophan in the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
    Illegal AgeI site found at 639
    Illegal AgeI site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/transporter
//chassis/prokaryote/ecoli
Parameters
chassisEscherichia coli
directionForward
functionTransporter
originEscherichia coli MG1655 - Codon optimized
targetTryptophan, Tyrosine, Phenylalanine