Coding

Part:BBa_K2380005

Designed by: Feodor Belov, Isabelle Feinauer, Sophia Hein, Tim Maier, Patrick Müller, Bea-Marie Spiekermann, Lara Steinel, Stefan Zens   Group: iGEM17_TU_Darmstadt   (2017-10-19)
Revision as of 13:46, 28 October 2017 by Th.wagner (Talk | contribs)


Chitinase A1

Chitinase A1 (ChiA1) is a hydrolase isolated from the organism Bacillus circulans WL-12. The chiA gene is 1983 base pairs long and translates into an enzyme with a molecular weight of approximately 69 kDa. ChiA1's main function is to break down glycosidic bonds between N-Acetylglucosamine (GlcNAc) units in a chitin oligomer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
    Illegal AgeI site found at 952
    Illegal AgeI site found at 1240
    Illegal AgeI site found at 1303
    Illegal AgeI site found at 1366
    Illegal AgeI site found at 1696
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None