Reporter

Part:BBa_K2448004:Experience

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 12:52, 28 October 2017 by Brumake (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2448004

mCherry is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching. Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.

Our team used this codon optimized mCherry to build the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024) and test our Psicose Biosensors (BBa_K2448025, BBa_K2448026, BBa_K2448027, BBa_K2448028, BBa_K2448029, BBa_K2448030 and BBa_K2448031) as well as our Fructose Biosensor (BBa_K2448032).

User Reviews

UNIQ02ddf76d0c4ba51b-partinfo-00000000-QINU UNIQ02ddf76d0c4ba51b-partinfo-00000001-QINU